منابع مشابه
Mechanism for priming DNA synthesis by yeast DNA Polymerase α
The DNA Polymerase α (Pol α)/primase complex initiates DNA synthesis in eukaryotic replication. In the complex, Pol α and primase cooperate in the production of RNA-DNA oligonucleotides that prime synthesis of new DNA. Here we report crystal structures of the catalytic core of yeast Pol α in unliganded form, bound to an RNA primer/DNA template and extending an RNA primer with deoxynucleotides. ...
متن کاملEvidence that Errors Made by DNA Polymerase α are Corrected by DNA Polymerase δ
Y.I. Pavlov, C. Frahm, S.A. Nick McElhinny, A. Niimi, M. Suzuki, and T.A. Kunkel* Eppley Institute for Research in Cancer Department of Biochemistry and Molecular Biology Department of Pathology and Microbiology University of Nebraska Medical Center Omaha, Nebraska 68198 Laboratory of Structural Biology and Laboratory of Molecular Genetics National Institute of Environmenal Health Sciences Nati...
متن کاملExploration of factors driving incorporation of unnatural dNTPS into DNA by Klenow fragment (DNA polymerase I) and DNA polymerase α
In order to further understand how DNA polymerases discriminate against incorrect dNTPs, we synthesized two sets of dNTP analogues and tested them as substrates for DNA polymerase alpha (pol alpha) and Klenow fragment (exo-) of DNA polymerase I (Escherichia coli). One set of analogues was designed to test the importance of the electronic nature of the base. The bases consisted of a benzimidazol...
متن کاملPolymerase manager protein UmuD directly regulates Escherichia coli DNA polymerase III α binding to ssDNA
Replication by Escherichia coli DNA polymerase III is disrupted on encountering DNA damage. Consequently, specialized Y-family DNA polymerases are used to bypass DNA damage. The protein UmuD is extensively involved in modulating cellular responses to DNA damage and may play a role in DNA polymerase exchange for damage tolerance. In the absence of DNA, UmuD interacts with the α subunit of DNA po...
متن کاملDistinct Double- and Single-Stranded DNA Binding of E. coli Replicative DNA Polymerase III α Subunit
The alpha subunit of the replicative DNA polymerase III of Escherichia coli is the active polymerase of the 10-subunit bacterial replicase. The C-terminal region of the alpha subunit is predicted to contain an oligonucleotide binding (OB-fold) domain. In a series of optical tweezers experiments, the alpha subunit is shown to have an affinity for both double- and single-stranded DNA, in distinct...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)83733-4